BD human CD16 PE-CY7 MAB抗體

英文名：PE-Cy™7 Mouse Anti-Human CD16

克隆號：Clone  3G8  (RUO)

BD human CD16 PE-CY7 MAB抗體

介紹：

Brand

BD Pharmingen™

Vol. Per Test

5 µl

Isotype

Mouse BALB/c x DBA/2, also known as CD2F1 or CDF1 IgG1, κ

Reactivity

Human (QC Testing)  Rhesus, Cynomolgus, Baboon (Reported) 

Application

Flow cytometry (Routinely Tested) 

Immunogen

Human polymorphonuclear leukocytes

Workshop No.

IV N409

Entrez Gene ID

2214  2215 

Storage Buffer

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status

RUO

規(guī)格

 100 Tests 25 Tests 

貨號

557744

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DESCRIPTION

The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al., 1997) to increase intracellular calcium levels in human neutrophils by interacting with bothFcγRIIa and FcγRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.

FORMAT

Format

PE-Cy™7

Excitation Source

Blue 488 nm,Green 532 nm,Yellow/Green 561 nm

Excitation Max

496 nm

Emission Max

785 nm

PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.

Other Formats →

• APC
• APC-Cy™7
• APC-H7
• Alexa Fluor® 647
• Alexa Fluor® 700

• BUV395
• BUV496
• BUV737
• BV421
• BV510

• BV605
• BV650
• BV711
• BV786
• Biotin

• FITC
• NA/LE
• PE
• PE-CF594
• PE-Cy™5

SUGGESTED COMPANION PRODUCTS

RESOURCES & TOOLS

• Spectrum Viewer
• Panel Designer

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PREPARATION AND STORAGE

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.